Quantitative PCR (qPCR) Assays and Instruments Market 2013 Trends

2013-04-29
Published : Apr-2013

This market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on real-time quantitative PCR (qPCR) assays and instruments carried out in March 2013. The study was initiated by HTStec as part of its ongoing tracking of emerging life science technologies and marketplaces. The main objectives were to comprehensively document current practices, preferences and metrics of qPCR assays, reagents and thermal cycling instruments. The survey also examined possible interest in a new chip-based qPCR platform enabling faster lower volume qPCR assays.

The report (http://www.reportsnreports.com/reports/238030-qpcr-assay-trends-2013.html) survey looked at the following aspects of qPCR assays and instruments as practiced today (2013) and predicted for the future (2015):

  • level of use and main type of qPCR activity
  • main applications of qPCR investigations
  • types of analysis performed on qPCR results
  • main origin of nucleic acid analyzed
  • main sources for nucleic acids processed
  • nucleic acid extraction/preparation techniques currently used
  • typical volume of samples after nucleic acid extraction
  • number of qPCR reactions set up per year and number of replicates qPCR reactions/sample
  • number of qPCR reactions processed in the average processing run and number of runs/working day
  • main qPCR format used; level of multiplexing used
  • typical total reaction volume used in qPCR assays
  • typical cost per reaction (data point) for qPCR assay chemistry/reagents and PCR-specific consumable labware
  • annual budget for qPCR assay consumables and its component breakdown
  • preferred suppliers of qPCR reagent chemistries
  • number of qPCR thermal cycling instruments per lab
  • manufacturer’s qPCR thermal cyclers most used
  • plans to purchase new qPCR real-time cyclers
  • most appealing new qPCR real-time thermal cycler manufacturer
  • type of sample prep instrumentation used to add cDNA and mastermixes
  • aspects of HT qPCR assays that are automated
  • biggest unmet market needs in qPCR assay setup and thermal cycling instruments
  • most important attributes when purchasing new qPCR thermal cycling instrumentation
  • relative importance of increasing throughput, decreasing analysis time and lowering reagents consumption (price/assay)
  • main applications for a new qPCR thermal cycling platform enabling faster and cheaper assays
  • interest in using a non-microplate chip format if it enabled faster and cheaper qPCR assays
  • factors of greatest influence in adopting a nonmicroplate chip format
  • advantage of having reagents (PCR mastermixes) pre-dispensed (immobilized) into a chip format
  • interest in receiving more information or evaluating a new real-time chip-based qPCR analyzer.

 

The main questionnaire consisted of 33 multi-choice questions. In addition, there were 8 questions related solely to the administration and demographics of survey. The survey collected 205 validated responses, of these 83% provided comprehensive input. Survey respondents met the following criteria:

1) were currently undertaking qPCR assays

2) make or influence purchasing decisions for qPCR reagents, kits or consumables.

Survey responses split geographically:

  • 41% Europe
  • 39% North America
  • 12% Asia (excluding Japan)
  • 5% Rest of World
  • 2% South America
  • 1% Japan

Respondents came from 67 Universities; 43 Research Institutes; 22 Hospitals/Clinics/Medical Schools; 19 Biotechs; 18 Government Labs; 9 Pharma; 8 Diagnostics; 6 Others; 5 Veterinary Labs/Animal Biotechs; 3 Agri Biotechs/Plant Genomics; 3 Not-for-Profits; 1 CROs; 1 Military/Defense Facility; and 1 Food Safety Establishment.

Most survey respondents had a senior job role or position which in descending order, is: 43 research scientists; 32 principal investigators; 29 lab/research managers; 23 professors/assistant professors; 21 senior scientists/research associates; 20 post-docs; 10 section/group leaders; 8 others; 4 directors; 2 department heads; and 1 vice president.

Survey results were expressed as an average of all survey respondents. In addition, the data was fully reanalyzed after sub-division into the following 4 survey groups: 1) academic research; 2) all other organizations; 3) mainly use standardized assays; and 4) mainly use custom assays.

The majority of respondents were making moderate use (1K-25K reactions/year) of qPCR today. The main type of qPCR activity run by respondents was evenly split between standardized and custom assays. The main application of qPCR investigations was gene expression analysis. The type of analysis mainly performed on real-time qPCR results today was relative quantitation.

mRNA was ranked the main origin of nucleic acids analyzed by qPCR. Human was the main source of nucleic acids processed by qPCR. The nucleic acid extraction/preparation techniques most used for qPCR setup was column-based purification. A median of 50-100μL was the typical volume of samples after nucleic acid extraction. A median of 1K-5K qPCR reactions were setup per lab per year today. A median of 2 replicate qPCR reactions were performed per sample today. A median of 50-100 qPCR reactions were processed per average run today. A median of 2 qPCR runs were made per working day today. The main qPCR assay format used today was the 96-well plate. A median of 1 dye was used per qPCR reaction today (i.e. multiplexing not routinely performed). A median of 25-50μL was the typical total reaction volume used in qPCR assay processing today. A median cost of $0.50-$1.00 per qPCR reaction was spent on assay chemistry/reagents today. A median cost of $0.20-$0.50 per qPCR reaction was spent on PCR-specific consumable labware today.

A median budget of $10K-$25K per lab per year was allocated for qPCR assay consumables today. The biggest share of this budget was allocated to commercial qPCR master mixes. The global market for qPCR assay consumables was estimated to be around $600M in 2013. The preferred supplier of qPCR reagent chemistries was Life Technologies. A median of 2 qPCR thermal cycling instruments were actively used in respondent’s lab today. The thermal cycler most used in respondent’s lab today was from ABI (Life Technologies). A median annual capex budget of $10K-$25K per lab per year was allocated for real time qPCR thermal cyclers today. This budget was expected to purchase a median of 1 unit.

The global market for qPCR thermal cyclers was estimated to be around $120M in 2013. The top 3 real time qPCR thermal cyclers respondents find most appealing to purchase were from ABI (Life Technologies), Bio-Rad and Roche. The sample prep instrumentation most used by respondents for adding cDNA or mastermixes when setting up qPCR assays was none i.e. manual hand pipettors were used. Most of these came mainly from Eppendorf and Gilson.

Most respondents have applied automation to some liquid handling steps in qPCR assays today. Reduce the price of qPCR reagents was rated as the most important unmet market need in qPCR assay setup and thermal cycling instrument requirements. Instrument reliability was rated the most important decision factor when evaluating qPCR thermal cycling instrumentation for purchase. Lowering reagents consumption (price/assay) was ranked as the most important feature when considering any new qPCR thermal cycling platform. Respondents could manage most without decreasing analysis time and increasing throughput in a new qPCR thermal cycling platform provided lowering reagents consumption (price/assay) was enabled. The application which respondents could most advantageously pursue on a new qPCR thermal cycling platform was gene expression analysis. Most respondents were interested in a using a non-microplate chip format that enabled faster and cheaper qPCR assays. Chip pricing was ranked the greatest influence on the purchasing of a non-microplate chip format. Most respondents thought it was highly advantageous to have reagents predispensed into a chip format.

Interest in a new chip-based qPCR platform was greatest for a 96-microreactor chip, with median reasonable pricing of $10/empty chip or $25/chip containing preimmobilized qPCR assay reagents/mastermixes. 71% of respondents were interested to receive more information about a new chip-based qPCR platform. 39% of respondents were interested to evaluate a new chip-based qPCR platform.

The full report (http://www.reportsnreports.com/reports/238030-qpcr-assay-trends-2013.html) provides the data, details of the breakdown of the responses for each question, its segmentation and all the estimates for the future (2015). It also highlights a few interesting differences between the survey groups. Contact [email protected] for further information.

 

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